mrna stability การใช้

• The result is loss of function, which affects mRNA stability.
• M 6 A methylation regulates nuclear export of mature mRNA and mRNA stability.
• Rbfox1 cytoplasmic variants modulate mRNA stability and translation.
• When used in the 3'UTR of a mammalian expression cassette it can significantly increase mRNA stability and protein yield.
• Multiple mutations have been found in different families, including those that molecularly disrupts the protein dimerization interface or reduces mRNA stability.
• It is currently unclear whether the changes in the level of AGAT transcript results from altered mRNA stability or enhanced transcriptional rate.
• Thiolutin is also an inhibitor of mannan and glucan formation in " Saccharomyces cerevisiae " and used for the analysis of mRNA stability.
• BACE1-AS regulates the expression of BACE1 by increasing BACE1 mRNA stability and generating additional BACE1 through a post-transcriptional feed-forward mechanism.
• It has also shown to be a neuronal activity response factor in the dendrites of hippocampal neurons suggesting possible roles in regulating mRNA stability, transport and local translation in neurons.
• They have a highly conserved 3'UTR and contain tandem repeat sequences at the 5'flank and 3'UTR which are thought to play a role in regulation of the mRNA stability.
• The N172 residue ( as part of the YMNM ) is important for the binding of Grb2 and Gads and seems to be able to induce IL-2 mRNA stability but not NF-?B translocation.
• Additionally, IL-10 expression is extensively regulated at the post-transcriptional level, which may involve control of mRNA stability via AU-rich elements and by microRNAs such as let-7 or miR-106.
• For example, a SNP both within the truncated form of ZFAT and the promoter of an antisense transcript increases the expression of ZFAT not through increasing the mRNA stability, but rather by repressing the expression of the antisense transcript.
• Proteins that bind to either the 3'or 5'UTR may affect translation by influencing the ribosome's ability to bind to the mRNA . MicroRNAs bound to the 3'UTR also may affect translational efficiency or mRNA stability.
• These regions are transcribed with the coding region and thus are exonic as they are present in the mature mRNA . Several roles in gene expression have been attributed to the untranslated regions, including mRNA stability, mRNA localization, and translational efficiency.
• "' Polypyrimidine tract-binding protein "'is an RNA-binding protein, also known as PTB or hnRNP I . PTB functions mainly as a splicing regulator, although it is also involved in alternative 3'end processing, mRNA stability and RNA localization.
• Eukaryotic RNA must undergo a series of modifications in order to be exported from the nucleus and successfully translated into function proteins, many of which are dependent on mRNA capping, the first mRNA modification to take place . 5'capping is essential for mRNA stability, enhancing mRNA processing, mRNA export and translation.
• It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA . The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
• In Molecular biology, the "'Iron response element "'or "'Iron-responsive element "'( "'IRE "') is a short conserved stem-loop which is bound by 3'UTR of the transferrin receptor ( involved in iron acquisition ) leads to increased mRNA stability.
• A shared feature of these proteins is to bind only to a subclass of the known AREs that contain the pentamer AUUUA . A convergent effort of several research teams now adds CUGBP1 ( CUG binding protein 1 ) to the short list of ARE-Binding proteins that control mRNA stability, with the peculiarity that it binds to non-AUUUA AREs.